Coding

Part:BBa_K1456003:Experience

Designed by: safa tapan   Group: iGEM14_ATOMS-Turkiye   (2014-07-21)
Revision as of 12:53, 21 October 2014 by Mustafa26 (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1456003

SOD ASSAY

  • Superoxide Dismutase 1 changes the oxygen radicals into 02 and H2O2. Using this feature of SOD we placed the oxygen radicals into the same solution as tetrazolium dye which uses the radicals as substrates and enabled them to compete. Hence this allowed us to measure the functional activity of SOD produced by our transfected cells using the spectrophotometer at 560 nm.
ATOMS-SOD2.jpg


ATOMS-SOD6.jpg


User Reviews

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