Part:BBa_K1321334:Experience

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Applications of BBa_K1321334

This part was cloned into part BBa_K1321333 by Biobrick cloning. The destination vector, containing the AraC-pBAD regulatory elements, was linearized using SpeI and PstI and AcsAB (previously digested with XbaI and PstI, was ligated at a 1:1 using the T4 ligase. The ligation mix was transformed into chemically competent DH10B Escherichia coli and plated on LB Agar+Chloramphenicol plates, grown overnight at 37 degrees celsius. 5 ml LB falcons supplied with 50 ug/ml Chloramphenicol were inoculated with a selection of freshly grown colonies for further restriction analysis (refer to Results for additional information)

User Reviews

UNIQ13951a5b526c192f-partinfo-00000000-QINU UNIQ13951a5b526c192f-partinfo-00000001-QINU