Part:BBa_K1321304
pSEVA321-BB with J23117-RFP
This part contains Anderson promoter J23117 and RFP coding device in pSEVA321-BB backbone. pSEVA321-BB is a broad host range plasmid, capable of replication in E.coli and several other G- bacterial species. We have verified that it is also capable of replication in the cellulose producing bacterium Gluconacetobacter xylinus. We have used this part to express RFP in G.xylinus.
BBa_K1321304 is a member of the G.xylinus genetic engineering toolbox. G.xylinus toolbox was created to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium Gluconacetobacter xylinus (parts Bba_K1321305 and BBa_K1321306) by testing out and providing a collection of widely used parts in pSEVA331-Bb backbone. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in G.xylinus and E.coli (a shuttle vector) and was selected because the registry's standard plasmid backbone pSB1C3 can not be used for G.xylinus engineering.
NOTE: Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in Gluconacetobacter species, the G.xylinus genetic engineering toolbox is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which can't be quality controlled and thus maintained by the registry. However, in order to make the G.xylinus toolbox available for the synthetic biology community, Imperial iGEM 2014 team has made the toolbox freely available upon request, with quality control provided (see Experience). For requests, please contact Imperial iGEM 2014 team.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4476
Illegal SpeI site found at 37
Illegal PstI site found at 920 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4476
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37
Illegal PstI site found at 920
Illegal NotI site found at 913
Illegal NotI site found at 4482 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4476
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4476
Illegal SpeI site found at 37
Illegal PstI site found at 920 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4476
Illegal XbaI site found at 4491
Illegal SpeI site found at 37
Illegal PstI site found at 920
Illegal NgoMIV site found at 2095
Illegal NgoMIV site found at 2980
Illegal NgoMIV site found at 4091
Illegal NgoMIV site found at 4215
Illegal AgeI site found at 616
Illegal AgeI site found at 728 - 1000COMPATIBLE WITH RFC[1000]
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