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UNIQfaf76ac80c19f706-partinfo-00000000-QINU UNIQfaf76ac80c19f706-partinfo-00000001-QINU The TMM enzyme is not specific to TMA as a substrate. It is also known to oxidize indole to indoxyl, which dimerizes into the well known blue pigment indigo. Indole is a natural product of tryptophan metabolism in E. coli. We took advangage of this indole production activity to characterize the TMM enzyme. E. coli that were cultured in LB supplemented with tryptophan (2 g/L) produced a deep blue pigment with absorbance properties matching indigo (Fig. 1). Without TMM expression or without tryptophan, indigo production was minimal or absent.

Figure 1. Average absorbances of DMSO extractions from bacterial lysates shows TMM activity.

GC/MS confirmed the activity of TMM by proving the degradation of trimethylamine (Fig. 2). It was performed on extractions from cultures of TMM-expressing E. coli (TMM) and control expressing an empty vector in a LB medium supplemented with trimethylamine (1 mM). The results show a significant decrease (p-value = 0,0199) of the concentration of TMA in TMM-expressing E.coli (Fig. 5D). This confirms the efficiency of degradation of the fish odor by TMM.

Figure 2. GC/MS analysis of cultures of E. coli expressing TMM or an empty vector (control).