Translational_Unit

Part:BBa_K1321334:Design

Designed by: Laura de Arroyo Garcia   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 11:29, 20 October 2014 by Ld1411 (Talk | contribs) (→‎Design Notes)


pSB-AcsAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1831
    Illegal BglII site found at 2545
    Illegal BglII site found at 4468
    Illegal BamHI site found at 841
    Illegal BamHI site found at 3835
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1817
    Illegal AgeI site found at 1894
    Illegal AgeI site found at 2450
    Illegal AgeI site found at 3251
    Illegal AgeI site found at 3343
    Illegal AgeI site found at 3449
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Ribosomal binding site of AcsAB is already included within the sequence. Because we are transferring this element from Gluconacetobacter into simpler organisms, such as E.coli, the native RBS was replaced with a new RBS, that would guarantee strong translation whilst maintaining the native stoichiometry for the final operon system to operate successfully.

Source

The AcsAB coding sequence was extracted from NCBI, codon optimised and edited accordingly for further gene synthesis and cloning into pSB1C3.

References