Protein_Domain

Part:BBa_K1462502

Designed by: zhanru Chen   Group: iGEM14_SCUT   (2014-10-14)
Revision as of 15:05, 19 October 2014 by Firedfish (Talk | contribs)

PDZ domain + Linker

The PDZ domain, one of our scaffold subunits, is a part of the Crk protein in Mus musculus.. Now we do the codon opmitization according to our host cell S.cerevisae. The SH3 domain allows the assembly of our targeted protein RuBisCo. And the domain is the second subunit of our scaffold protein.

[[File:SH3.png|200px|left|frame|Simulative structure of the SH3 domain which we add the linker behind it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 310
    Illegal XhoI site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None