Coding

Part:BBa_K1331002

Designed by: Jiayi Dong   Group: iGEM14_Nankai   (2014-09-25)
Revision as of 04:35, 19 October 2014 by Danielle D (Talk | contribs)

RhlR regulatory protein from P. aeruginosa SQ6

This part encodes a transcription regulatory protein RhlR.

It includes the whole coding sequence and a part of downstream noncoding sequence of RhlR.

The purpose of remaining the noncoding sequence is for composition with rhlI. See our composite part rhlRI (BBa_K1331007).


Usage and Biology

RhlR is necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase I.

It also functions as a transcriptional activator of elastase structural gene (lasB).

RhlR binds to autoinducer molecules C4-HSL which is synthesized by rhlI, and the complex positively regulates the promoter of rhlAB consequently, while rhlR alone negtively regulates the rhlAB promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 863
    Illegal BamHI site found at 344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 843
    Illegal NgoMIV site found at 847
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//cds/transcriptionalregulator
//chassis/prokaryote/ecoli
Parameters
None