Coding

Part:BBa_K1331003

Designed by: Jiayi Dong   Group: iGEM14_Nankai   (2014-09-26)
Revision as of 04:32, 19 October 2014 by Danielle D (Talk | contribs)

RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6

This part encodes rhlI, an acyl-homoserine-lactone synthase in Pseudomonas aeruginosa.

The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence.

Usage and Biology

The sequence we provide here includes the whole coding sequence and a part of upstream noncoding sequence of rhlI.

RhlI is an acyl-homoserine-lactone synthase required for the synthesis of BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone) autoinducer molecules which bind to RhlR. RhlI is required for the synthesis of autoinducer molecules C4-HSL.Together with rhlR positively regulates the promoter of rhlAB.

The noncoding sequence included in this part is for composition with rhlR. See our composite part rhlRI (BBa_K1331007).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 89
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 221


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
Parameters
None