Coding

Part:BBa_K1403019

Designed by: Paris Bettencourt 2014   Group: iGEM14_Paris_Bettencourt   (2014-10-06)
Revision as of 04:16, 19 October 2014 by Tovila (Talk | contribs)

Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette

  • Constitutive T7 promoter
  • RBS
  • CDS: IlvB/N from E. coli MG1655

Transform E. coli to overexpress ilvB and thus increase the basal production of alpha acetolactate, the precursor of acetoin.

  • This was submitted in a pETDuet-1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None