Generator

Part:BBa_K1403017

Designed by: Sylvia   Group: iGEM14_Paris_Bettencourt   (2014-10-06)
Revision as of 04:15, 19 October 2014 by Tovila (Talk | contribs)

Acetaldehyde dehydrogenase (aldB) expression cassette

Transform E. coli to produce acetoin.
AldB requires alpha acetolactate as substrate. A co-transformation with part BBa_K1403019 increases the amount of intracellular alpha acetolactate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Verification

AldB.png

Expeceted bands at 2kbp for pSB1C3 and 846bp for aldB gene with promoter

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Categories
Parameters
None