Generator

Part:BBa_K1403009

Designed by: Paris Bettencourt 2014   Group: iGEM14_Paris_Bettencourt   (2014-10-06)
Revision as of 04:11, 19 October 2014 by Tovila (Talk | contribs)

Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) expression cassette

  • Constitutive promoter BBa_J23108
  • Synthetic RBS
  • CDS: BSMT1 from Petunia × hybrida
  • Histidine Tag

Transform E. coli and grow in the presence of salicylic acid or benzoic acid for production of methyl salycilate or methyl benzoate respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None