Coding

Part:BBa_K1331001

Designed by: Tianyi Huang   Group: iGEM14_Nankai   (2014-09-25)
Revision as of 03:40, 19 October 2014 by Asphodel19150 (Talk | contribs) (→‎Usage and Biology)

Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6


This is an improvement of BBa_K317998 designed by iGEM10_Tokyo-NoKoGen. A mutation has been done to remove the PstI restriction site (at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Usage and Biology

The sequence we provide here includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.

RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa.This part encodes rhamnosyltransferase I subunit A, and the other part BBa_K1331004 encodes subunit B.

The noncoding sequence included in this part is for composition with rhlB. The downstream noncoding sequence of rhlA and The upstream noncoding sequence of rhlB (BBa_K1331004) make the whole noncoding sequence between rhlA and rhlB in Pseudomonas aeruginosa, preserving the overall function of the compositive part. We also built this compositive part rhlAB, see BBa_K1331006.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
    Illegal BamHI site found at 629
    Illegal XhoI site found at 805
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 294
    Illegal BsaI.rc site found at 478


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
Parameters
None