Coding

Part:BBa_K1502001:Design

Designed by: Matthew Ykema   Group: iGEM14_Arizona_State   (2014-10-17)
Revision as of 23:47, 18 October 2014 by Kahaynes (Talk | contribs) (→‎Source)

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accA portion of Acyl-CoA Ligase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 517
    Illegal SapI.rc site found at 714


Design Notes

The PCR process included X and P overhangs in order to include into the pSB1C3 plasmid.


Source

This part was isolated through PCR from the pACM4-ACA plasmid ordered from AddGene (#49809).

References

(2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Eric J. Steen, Yisheng Kang, Gregory Bokinsky, Zhihao Hu, Andreas Schirmer, Amy McClure, Stephen B. del Cardayre and Jay D. Keasling. Nature. 275: 28593-28598.