Generator
Part:BBa_K1403017
Designed by: Sylvia Group: iGEM14_Paris_Bettencourt (2014-10-06)
Acetaldehyde dehydrogenase (aldB) expression cassette
- Constitutive promoter BBa_J23108
- Synthetic RBS
- CDS: AldB from Lactococcus lactis
- Terminators BBa_B0010 & BBa_B0012
Transform E. coli to produce acetoin. The enzyme requires alpha acetolactate. A co-transformation with part BBa_K1403019 increases the amount of substrate for aldB.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000COMPATIBLE WITH RFC[1000]
Verification
Expeceted bands at 2kbp for pSB1C3 and 846bp for aldB gene with promoter
[edit]
Categories
Parameters
None |