Regulatory

Part:BBa_K1526009:Experience

Designed by: Hanna Esser   Group: iGEM14_York   (2014-10-07)
Revision as of 16:14, 18 October 2014 by Gyaros (Talk | contribs) (→‎User Reviews)


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Please enter how you used this part and how it worked out.

Creating the regulator LacRS

In order to characterize the parts we have created a new BioBrick compatible with our chassis. As E.coli DH5α does not have a lac repressor system we have assembled together basic parts J32100, B0033, C0012, B0010, B0012 and R0010 to create the composite regulator Lac Repressor System (LacRS)

<img src="York_Construct.PNG" style="border:1px solid orange">

Our first functionalization test coupling LacRS upstream the Green Fluorescent Protein (GFP) Biobrick (E0040) showed expression of GFP under UV light when the system is previously induced by IPTG.

We have successfully cloned and tested the functionality of LacRS in pSB1A3 (<a href="https://parts.igem.org/Part:BBa_K1526009">BBa_K1526009</a>) into E.coli DH5α

<img src=Plates_LacRS_I.jpg class="img-responsive" style="width:100%; border:3px solid orange;">

User Reviews

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