Coding
Part:BBa_K1403019
Designed by: Paris Bettencourt 2014 Group: iGEM14_Paris_Bettencourt (2014-10-06)
Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette
- Constitutive T7 promoter
- RBS
- CDS: Acetohydroxybutanoate synthase/acetolactate synthase (IlvB/N) from E. coli MG1655
Transform E. coli to overexpress ilvB and thus increase the basal production of alpha acetolactate, the precursor of acetoin.
- This was submitted in a pETDuet-1
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 47
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 47
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |