Translational_Unit

Part:BBa_K1526003

Designed by: Cauă Westmann   Group: iGEM14_York   (2014-10-07)
Revision as of 15:43, 18 October 2014 by Gyaros (Talk | contribs)

CysP from Bacillus subtilis

The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family

Gene: cysP (AKA YlnA)
Organism: Bacillus Subtilis
Protein: CysP
Function: This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space.
Aim: We want to over-express this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium.
Expression: We could run assays measuring the concentration of sulfate in the environment containing our bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 680
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None