Generator

Part:BBa_K1403012

Designed by: Paris Bettencourt 2014   Group: iGEM14_Paris_Bettencourt   (2014-10-06)
Revision as of 14:49, 18 October 2014 by Tovila (Talk | contribs)

Alcohol acetyltransferase I (ATF1) expression cassette

  • Constitutive promoter BBa_J23108
  • RBS BBa_B0030
  • CDS: Alcohol acetyltransferase I (ATF1)
  • Terminators BBa_B0010 & BBa_B0012

Transform E. coli and grow in the presence of isoamyl alcohol to produce isoamyl acetate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None