Generator

Part:BBa_K1403006

Designed by: Antonio Villarreal Larrauri   Group: iGEM14_Paris_Bettencourt   (2014-10-06)
Revision as of 13:57, 18 October 2014 by Tovila (Talk | contribs)

Jasmonic acid carboxyl methyltransferase (JMT) expression cassette

  • Constitutive promoter BBa_J23108.
  • Synthetic RBS
  • CDS: Jasmonic acid carboxyl methyltransferase (JMT)
  • His Tag

Transform E. coli for Methyl jasmonate biosynthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 222
    Illegal BamHI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]



1 mM Jasmonic acid

[edit]
Categories
Parameters
None