DNA

Part:BBa_K1431411

Designed by: Fan Jiang, Peng Peng   Group: iGEM14_SUSTC-Shenzhen   (2014-10-14)
Revision as of 03:56, 18 October 2014 by Brando (Talk | contribs)

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target sequence for HIV


Introduction

This three plasmid(with BBa_K1431412 and BBa_K1431413) carry the Target-sequence aim to check if the CRISPR-Cas9 system we create in human cell can work well, which might be the foundation for the further experiments. .

However, although each one with different target-sequence, they still have plenty of components in common. You can see, all of them carry at least one section of target-sequences, and target-sequence for HBV1-HBV2 have two sections of target-sequence.This is because this part plays an important role in efficiency test, both for target-sequence binding gRNA and cut-off ability of CRISPR-Cas9.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None