Coding

Part:BBa_J36835:Design

Designed by: Perry Tsai   Group: iGEM06_Harvard   (2006-10-29)
Revision as of 21:12, 29 October 2006 by Perrytsai (Talk | contribs) (→‎Design Notes)

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Lipoprotein signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.

Source

E. coli K12 genome

References