Composite

Part:BBa_J36838:Design

Designed by: Perry Tsai   Group: iGEM06_Harvard   (2006-10-29)
Revision as of 20:42, 29 October 2006 by Perrytsai (Talk | contribs) (→‎Design Notes)

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Lipoprotein signal peptide + Outer membrane protein A, aa 46-66


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.

Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts.

Source

Assembly of BBa_J36835 and BBa_J36836

References