Composite

Part:BBa_K1491016

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-08-04)
Revision as of 03:02, 18 October 2014 by Lenaw (Talk | contribs)

Lac Inducible Codon Optimized KillerRed

Codon optimized KillerRed with WTLac promoter allows for induction of KillerRed expression with IPTG

It has been optimized for E. coli by eliminating the rare codons.

Characterization:

The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm)

800px-Colonies.jpg

736px-RFU.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 583


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