Coding
tetA(C)b

Part:BBa_J31006:Design

Designed by: Sabriya Rosemond, Erin Zwack   Group: iGEM06_Davidson   (2006-07-12)
Revision as of 17:22, 29 October 2006 by Kahaynes (Talk | contribs)

tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 897
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 503
    Illegal NgoMIV site found at 871
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa J31006 was cloned into vector pSB1A2. The Biobricks on this part are not wild type, but the cut sites are still viable.

Standard BioBrick Cloning Sites (Knight) 5'--GAATTC GCGGCCGC T TCTAGA G ----insert---- T ACTAGT A GCGGCCG CTGCAG--
3'--CTTAAG CGCCGGCG A AGATCT C -------------- A TGATCA T CGCCGGC GACGTC--
BBa_J31001 Cloning Sites 5'--GAATTC GCGGCCGC T TCTAGA * --Tet coding-- * ACTAGT A GCGGCCG CTGCAG--
3'--CTTAAG CGCCGGCG A AGATCT * -------------- * TGATCA T CGCCGGC GACGTC--

Prefix
There is no G spacer (*) between the XbaI and the Hin coding region.
Suffix
There is no T spacer (*) between the Hin coding region and the SpeI site.

Data

Once placed to the right of a reverse RBS, TetB conveys tetrcycline resistance to JM109 cells (see BBa_S03532).

Source

The tetracycline resistance coding region was PCR amplified from pSB1AT3.

References