Translational_Unit

Part:BBa_K1500010

Designed by: Rivka Baker Keusch   Group: iGEM14_UChicago   (2014-10-17)
Revision as of 02:55, 18 October 2014 by RBeckner (Talk | contribs)

IPTG--GFP--EmrR

Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream with an additional RBS and the EmrR mutator gene. Using a fluctuation analysis mutation rate experiment, XL1Blue bacteria transformed with this construct yielded a mutation rate of 3.14E-09 mutations per cell per culture although mutation rate is dependent on growth conditions and can vary depending on experimental setup. This plasmid appears to incur a growth penalty in transformants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1424
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


[edit]
Categories
Parameters
None