Coding
tetA(C)b

Part:BBa_J31006:Design

Designed by: Sabriya Rosemond, Erin Zwack   Group: iGEM06_Davidson   (2006-07-12)
Revision as of 17:06, 29 October 2006 by Kahaynes (Talk | contribs)

tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 897
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 503
    Illegal NgoMIV site found at 871
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was cloned into PsB1A2 plasmids.

Biobricks

The Biobricks on this part are not wild type, but the cut sites are still viable.

Standard BioBrick Cloning Sites (Knight) 5'--GAATTC GCGGCCGC T TCTAGA G ----insert---- T ACTAGT A GCGGCCG CTGCAG--
3'--CTTAAG CGCCGGCG A AGATCT C -------------- A TGATCA T CGCCGGC GACGTC--
BBa_J31001 Cloning Sites 5'--GAATTC GCGGCCGC T TCTAGA * --Hin coding-- * ACTAGT T GCGGCCGCCTGCAG--
3'--CTTAAG CGCCGGCG * AGATCT * -------------- * TGATCA A CGCCGGCGGACGTC--

Prefix
There is no G spacer (*) between the XbaI and the Hin coding region.
Suffix
There is no T spacer (*) between the Hin coding region and the SpeI site.
The A spacer between the SpeI and the NotI has changed to a T.
There is an extra C between the NotI site and the PstI site

5'GAATTC GCGGCCGC T TCTAGA - aatccagctccaccgggccgaggtacgtggcgctgcgttgcgcccctccgtctgttccatatcccgccgcggatgttaggtacggttgggcaaggtacac gagcggctccgccgtatttagcggcactgctagtcgccaggttactagcttcaatccgaccattctcggcgctcgctaggaacttcgacagggactacca gcagtagatggacggacctgtcgtaccggacgttgcgcccgtagggctacggcggccttcgctcttcttagtattaccccttccggtaggtcggagcgca gcgcttgcggtcgttctgcatcgggtcgcgcagccggcggtacggccgctattaccggacgaagagcggctttgcaaaccaccgccccggtcactgcttc cgaactcgctcccgcacgttctaaggcttatggcgttcgctgtccggctagtagcagcgcgaggtcgctttcgccaggagcggcttttactgggtctcgc gacggccgtggacaggatgctcaacgtactatttcttctgtcagtattcacgccgctgctatcagtacggggcgcgggtggccttcctcgactgacccaa cttccgagagttcccgtagccagctgcgagagggaatacgctgaggacgtaatccttcgtcgggtcatcatccaactccggcaactcgtggcggcggcgt tccttaccacgtacgttcctctaccgcgggttgtcagggggccggtgccccggacggtggtatgggtgcggctttgttcgcgagtactcgggcttcaccg ctcgggctagaaggggtagccactacagccgctatatccgcggtcgttggcgtggacaccgcggccactacggccggtgctacgcaggccgcatctccta ggtgtcctgcccacaccagcggtactagcgcatcagctatcaccgaggttcatcgcttcgctcgtcctgacccgccgccggtttcgccagcctgtcacga ggctcttgcccacgcgtatctttaacgtagttgcgtatatcgcgatcgtcgtgcggtatcactgaccgctacgacagccttacctgctatagggcgttct ccgggccgtcatggccgtattggttcggatacggatgtcgtaggtcccactgccacggctcctactgctactcgcgtaacaatctaaagta - ACTAGT A GCGGCCG CTGCAG

EcoRI NotI Spacer XbaI Missing G spacer Coding Region Missing T spacer SpeI Spacer NotI PstI

We compared our BioBricks with those from Tom Knight's paper, Idempotent Vector Design for Standard Assembly of Biobricks. As seen below

BioBricks from paper.png

Source

PsB1AT3

References

Knight, Tom. Idempotent Vector Design for Standard Assembly of Biobricks