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Part:BBa_K1429002:Design

Designed by: Christal Gordon   Group: iGEM14_Genspace   (2014-09-29)
Revision as of 02:37, 18 October 2014 by EllenJ (Talk | contribs) (→‎Source)


Cyan Fluorescent Protein (CFP) "Cindy Lou" coding region, intellectual property-free


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 74
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


Design Notes

We started with the FPB-20-444 plasmid from DNA 2.0 which contains an "IP-Free" synthetic non-Aequorea CFP coding region downstream from a ribosome binding site (AGGAGG ). PCR primers were designed complementary to sequences flanking the RBS-CFP region, with a "tail" that added the biobrick prefix/suffix. The PCR product was digested with EcoR1 and Pst1 and ligated into the pSB1C3 plasmid backbone.

Source

DNA 2.0 Protein Paintbox

References