Translational_Unit

Part:BBa_K1429003:Experience

Designed by: Eric Fernandez   Group: iGEM14_Genspace   (2014-10-02)
Revision as of 02:24, 18 October 2014 by EllenJ (Talk | contribs) (→‎Applications of BBa_K1429003)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1429003

We have put this biobrick under the control of the T7 promoter by cloning the biobrick part into pUC19. The expression of the OFPYukon can be seen in the photograph. It is clone RFP2:

UV_light.jpg

20141015_174513.jpg

The lysis buffer from the Carolina Biological kit "Purification of Green Fluorescent Protein" was used to lyse cells from an overnight 2mL culture of E. coli K-12 Top10 cells containing pUC19 with this biobrick. The lysate was analyzed by fluorimetry to determine if the excitation and emission wavelengths matched the reported ones of 550 and 563 respectively. They did, confirming the protein was the correct one.

Ofp.jpg

User Reviews

UNIQc5a0eb9c13923d9d-partinfo-00000000-QINU UNIQc5a0eb9c13923d9d-partinfo-00000001-QINU