Plasmid
Part:BBa_K1391131:Design
Designed by: Shinjini Saha Group: iGEM14_MIT (2014-10-17)
pEXPR_TRE:miRNAG2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 5166
Illegal EcoRI site found at 5786
Illegal EcoRI site found at 5827
Illegal XbaI site found at 4525
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 6364
Illegal PstI site found at 7013 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 5166
Illegal EcoRI site found at 5786
Illegal EcoRI site found at 5827
Illegal NheI site found at 3210
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 6364
Illegal PstI site found at 7013
Illegal NotI site found at 6669 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 5166
Illegal EcoRI site found at 5786
Illegal EcoRI site found at 5827
Illegal BglII site found at 5907
Illegal BamHI site found at 2854
Illegal BamHI site found at 3328
Illegal BamHI site found at 4632
Illegal BamHI site found at 6864
Illegal XhoI site found at 3538
Illegal XhoI site found at 5062
Illegal XhoI site found at 6649 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 5166
Illegal EcoRI site found at 5786
Illegal EcoRI site found at 5827
Illegal XbaI site found at 4525
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 6364
Illegal PstI site found at 7013 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 5166
Illegal EcoRI site found at 5786
Illegal EcoRI site found at 5827
Illegal XbaI site found at 4525
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 6364
Illegal PstI site found at 7013
Illegal NgoMIV site found at 2297
Illegal NgoMIV site found at 3741
Illegal NgoMIV site found at 4024
Illegal AgeI site found at 3456 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1121
Illegal BsaI.rc site found at 5688
Illegal SapI site found at 38
Illegal SapI site found at 3214
Illegal SapI site found at 7096
Illegal SapI.rc site found at 4680
Design Notes
Some
Source
Artificial