Part:BBa_J63010

Protein fusion vector (Silver lab standard)

Standard biofusion plasmid containing ampillicin resistance

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3246
  Illegal XbaI site found at 3261
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3246
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  Illegal NotI site found at 8
  Illegal NotI site found at 3252
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3246
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Illegal EcoRI site found at 3246
  Illegal XbaI site found at 3261
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3246
  Illegal XbaI site found at 3261
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  Illegal NgoMIV site found at 2825
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1393
  Illegal SapI site found at 310