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Part:BBa_K1429002:Experience

Designed by: Christal Gordon   Group: iGEM14_Genspace   (2014-09-29)
Revision as of 02:22, 18 October 2014 by EllenJ (Talk | contribs) (→‎Applications of BBa_K1429002)


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Applications of BBa_K1429002

We have put this biobrick under the control of the T7 promoter by cloning the biobrick part into pUC19. The expression of the RFPRudolph can be seen in the photograph. It is clone CFP1:

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The lysis buffer from the Carolina Biological kit "Purification of Green Fluorescent Protein" was used to lyse cells from an overnight 2mL culture of E. coli K-12 Top10 cells containing pUC19 with this biobrick. The lysate was analyzed by fluorimetry to determine if the excitation and emission wavelengths matched the reported ones of 550 and 563 respectively. They did, confirming the protein was the correct one.

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