Plasmid
Part:BBa_K1391130:Design
Designed by: Shinjini Saha Group: iGEM14_MIT (2014-10-17)
pEXPR_TRE:miRNAG1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 5165
Illegal EcoRI site found at 5785
Illegal EcoRI site found at 5826
Illegal XbaI site found at 4525
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 6363
Illegal PstI site found at 7012 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 5165
Illegal EcoRI site found at 5785
Illegal EcoRI site found at 5826
Illegal NheI site found at 3210
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 6363
Illegal PstI site found at 7012
Illegal NotI site found at 6668 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 5165
Illegal EcoRI site found at 5785
Illegal EcoRI site found at 5826
Illegal BglII site found at 5906
Illegal BamHI site found at 2854
Illegal BamHI site found at 3328
Illegal BamHI site found at 4632
Illegal BamHI site found at 6863
Illegal XhoI site found at 3538
Illegal XhoI site found at 5062
Illegal XhoI site found at 6648 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 5165
Illegal EcoRI site found at 5785
Illegal EcoRI site found at 5826
Illegal XbaI site found at 4525
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 6363
Illegal PstI site found at 7012 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 5165
Illegal EcoRI site found at 5785
Illegal EcoRI site found at 5826
Illegal XbaI site found at 4525
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 6363
Illegal PstI site found at 7012
Illegal NgoMIV site found at 2297
Illegal NgoMIV site found at 3741
Illegal NgoMIV site found at 4024
Illegal AgeI site found at 3456 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1121
Illegal BsaI.rc site found at 5687
Illegal SapI site found at 38
Illegal SapI site found at 3214
Illegal SapI site found at 7095
Illegal SapI.rc site found at 4680
Design Notes
some
Source
Artificial