Composite

Part:BBa_K1491020

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-15)
Revision as of 02:17, 18 October 2014 by Lenaw (Talk | contribs)

Constitutive Codon Optimized Killer Red

Codon optimized version of superoxide generator Killer Red (BBa_K1491015) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034).

This part is the codon optimized version of KillerRed (BBa_K1184000). It has been optimized for E. coli by eliminating the rare codons.

Characterization:

The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm)

800px-Colonies.jpg

736px-RFU.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 418


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Categories
Parameters
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