Part:BBa_K1520520

PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter

PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter It is part of our test of suicide circuit by gold ions, BBa_K1520509 [1].

this is part of our part BBa_K1520509 [2] and the picture is also part of BBa_K1520509, since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.

From left to right, Marker(1Kb), PgolTS-golB-PgolB-tetR-Ter, PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter.

The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers. since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 548
  
• 1000
  COMPATIBLE WITH RFC[1000]