Composite

Part:BBa_K1431201

Designed by: Rifei Chen, Yushan Zhang   Group: iGEM14_SUSTC-Shenzhen   (2014-10-09)
Revision as of 01:54, 18 October 2014 by ChenRifei (Talk | contribs)

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NLS, lead protein into the nucleus

A protein tags with a nuclear localization signal or sequence(NLS) will bind strongly to importin (aka karyopherin), and, together, the complex will move through the nuclear pore.That is very useful to localize protein or make a sign in cell nucleus.

We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. See the mechanism of BbsI site work in the design page. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 66
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 88
    Illegal NgoMIV site found at 107
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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