Part:BBa_J58101

Promoter activated synergistically by cI and CRP with the reporter RFP

This composed part contains a two-regulator AND promoter activated synergistically by cI and CRP proteins:

pAND J58100

joined to a generator of the reporter RFP

I13507

The deep components are as follows:

pAND J58100	B0034	mRFP1 E1010	B0010	B0012

With this construction, we obtain a genetic device (not using POPS) that produces RFP when enough levels of cI and CRP are acheived simultaneously.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 11
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 11
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 11
  Illegal AgeI site found at 687
  Illegal AgeI site found at 799
  
• 1000
  COMPATIBLE WITH RFC[1000]

See comments in AND-type promoter synergistically activated by cI and CRP