![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1365000:Design
Designed by: Sandra Mous Group: iGEM14_Groningen (2014-09-23)
NisA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA-3'
to PCR NisA from the genome and add the BioBrick prefix and suffix.
Source
Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.
References
Trmčić, A. et al. (2011) Complete nisin A gene cluster from Lactococcus lactis M78 (HM219853) – obtaining the nucleic acid sequence and comparing it to other published nisin sequences. Genes Genom. 33: 217-221