Plasmid_Backbone
Part:BBa_J23006
Designed by: John Anderson Group: iGEM2006_Berkeley (2006-07-01)
Revision as of 04:50, 29 October 2006 by JCAnderson (Talk | contribs)
Part-making plasmid with Ptet-XbaI-[biobrick]-SpeI-PstI
pSB1A2-derived plasmid for constructing basic biobrick parts under the control of an external promoter. Insertion of parts between the XbaI and SpeI (or) PstI sites places the part under the control of the part r0040 (Tet) promoter. The resulting plasmid retains the normal sequence context of a r0040.newpart composite with one point mutation changing the ACTAGA linker to TCTAGA.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Illegal EcoRI site found at 2136
Illegal XbaI site found at 2350 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2136
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2142 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2136 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Illegal suffix found at 2
Illegal EcoRI site found at 2136
Illegal XbaI site found at 2350 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2136
Illegal XbaI site found at 2350
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1175
[edit]
Categories
Parameters
//plasmidbackbone/libraryscreening/rbscodingsequence
chassis | |
copies | |
mcs | BioBrick friendly |
origin | ColE1 |
resistance | A |