Composite
Part:BBa_K1462100
Designed by: Chuyao Fan Group: iGEM14_SCUT (2014-10-14)
Gal1+GFP+TOM22+ADH1
To verify the localization function of Tom22, the N-terminus of Tom22 was fused with BFP. We adopted fluorescence test to have a visible look at where the BFP is located. The following microscope images were captured. We can observe that the blue fluorescence concentrates in a specific area , instead of diffusing homogeneously in the cytosol.The reult proves that Tom22 can perform its function properly
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1468
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1468
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1468
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1468
Illegal AgeI site found at 150 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1199
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Categories
Parameters
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