Part:BBa_K1520008

Pcons2-rbs-lacI-Ter Pcons2-rbs-lacI-Ter. It constantly produces lacI.

From left to right, Marker (DL10000), Pcons2, Pcons2-lacI, Pcons-lacI-Ter.

Primers used in PCR are VF2 and VR

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1208
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]