Part:BBa_K1497005

B0034-CHS-B0034-CHI - Composite part of RBS-CHS and RBS-CHI

This device is able to generate naringenin from 4-courmaroyl-CoA, if put under a promotor. This device is the second composite part for the assembly of our naringenin operon (K1497007).

Part 1: BBa_K1497004: B0034-CHS - Chalcone Synthase from Gerbera with strong RBS The chalcone synthase catalyzes the reaction from 4-Coumaroyl-CoA to naringenin chalcone. It is one of the essential enzymes in the anthocyanin pathway.

Part 2: BBa_K1497003: B0034-CHI - Chalcone Isomerase from Petunia with strong RBS The chalcone isomerse catalyzes the reaction from naringenin chalcone to naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin.

Part 1 Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one naringenin calcone.

Part 2 Reaction of the CHI. A proton shift of naringenin chalcone builds naringenin. The CHI has a high E-value and produce only S-Naringenin.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1295
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1304
  Illegal XhoI site found at 302
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1275
  Illegal NgoMIV site found at 1851
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1224
  Illegal BsaI.rc site found at 706