Composite

Part:BBa_K1520024

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)
Revision as of 23:59, 17 October 2014 by Sagyman (Talk | contribs)

Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter

Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter It is a positive feedback plasmid using luxI and luxR as key to feedback. Introducing IPTG will produce rfp, luxI and luxR, and luxI together with luxR will work back to enhance production of rfp.

Positive feedback.jpg

The blue line in the picture shows the fluorescence intensity of the bacteria with this plasmid, which serves as a control in our experiment. The yellow line and purple line show the fluorescence intensity of the bacteria with different positive feedback plasmids.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2380
    Illegal NheI site found at 2403
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
    Illegal BglII site found at 2222
    Illegal BglII site found at 4818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3358


[edit]
Categories
//cds
//cds/transcriptionalregulator/activator
//cds/transcriptionalregulator/repressor
//function/cellsignalling
//function/regulation/transcriptional
Parameters
biology
controlluxR, HSL
directionForward
ec_numnone
functionMixed
keggnone
ligandsHSL
n/aconstitutive promoter family member
negative_regulators
o_h
o_l
positive_regulators1
proteinLuxR
swissproP12746
tagNone
uniprotP48445