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Composite

Part:BBa_K1463603

Designed by: iGEM14_Glasgow   Group: iGEM14_Glasgow   (2014-10-04)
Revision as of 23:54, 17 October 2014 by GemmaMcl (Talk | contribs)

FliC with RBS and J23106 promoter

FliC with B0032 and B0034 RBS under the control of J23106 promoter.


This composite part was made by inserting a synthesised double-stranded oligonucleotide containing J23106 promoter and B0032 into K1463601 (fliC and B0034 RBS).


As shown in Figure 1G and 1H, this construct in pSB1C3 was able to restore swimming in a fliC knockout strain to near wild-type levels. These results are also shown in Figure 2.


Flic Motility Swarm Assay

310px-GU_Figure_1_swarm_M.png

Figure 1: FliC Swarm Motility Assays.
(A) DS941, (B) DS941 ΔfliC,
(C) DS941 ΔfliC + pSB1C3 fliC (no promoter), (D) DS941 ΔfliC + J23100 (mutant promoter) fliC,
(E) DS941 ΔfliC + J23116-fliC(1), (F) DS941 ΔfliC + J23116-fliC(2),
(G) DS941 ΔfliC + J23106-fliC(1), (H) DS941 ΔfliC + J23106-fliC(2)


GU_Figure_2_Motility_histogram.png
Figure 2 - FliC Motility Histogram



For more information on the biobrick and methods used go to http://2014.igem.org/wiki/index.php?title=Team:Glasgow/Project/Mobility_Proteins#fliC


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 383
    Illegal AgeI site found at 791
  • 1000
    COMPATIBLE WITH RFC[1000]


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