Translational_Unit

Part:BBa_K1500015:Design

Designed by: Ryan Beckner   Group: iGEM14_UChicago   (2014-10-17)
Revision as of 23:20, 17 October 2014 by RBeckner (Talk | contribs) (→‎Design Notes)

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paroF->MCherry-MutH()


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1346
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mutagenized mutH was ligated into the paroF+mCherry-LVA construct plasmid

Source

paroF, mutH are genomic XL1Blue, mCherry is from distribution kit.

References