Coding
SYFP2

Part:BBa_K864100:Experience

Designed by: Erik Gullberg   Group: iGEM12_Uppsala   (2012-09-24)
Revision as of 23:08, 17 October 2014 by Dopeters (Talk | contribs) (Applications of BBa_K864100)

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Applications of BBa_K864100


iGEM14_Carnegie_Mellon. We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). YFP had a very high signal-to-noise ratio in MACH cells and a low signal-to-noise ratio in Top10 cells relative to other fluorescent proteins. YFP was measured at (ex/em = 514nm/527nm).

591px-FP_MACH.png 591px-Top10_Best.jpg


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iGEM Team Uppsala University 2012

This part was used as an fluorescent reporter in two ways by the Uppsala iGEM team 2012. It could easily be observed both in a Fluorescence activated cell sorter (FACS), on a UV table and even better on a Visi-Blue blue-light table. We recommend it whenever a strong fluorescent reporter is needed.

1. As a part of the sRNA screening system target construct (BBa_K864444), where it was a reporter of translational downregulation. Thanks to the very strong fluorescence, cells with very low levels of expression could easily be distinguished from non-fluorescent cells, using a FACS. In this experiment it was fused to a part of another protein, with the BBa_J18922 gly-ser linker in between. Fluorescence was not affected by this fusion.

2. In our promoter test, where it was coupled to different promoter as promoters-B0032-SYFP2 (using BBa_K864101).

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