Plasmid

Part:BBa_K1416004

Designed by: Jordan Monk, Colin Brown, Dennis Mishler   Group: iGEM14_Austin_Texas   (2014-10-17)
Revision as of 22:28, 17 October 2014 by Registry (Talk | contribs)

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pFRY: a Reporter Plasmid for Measuring ncAA Incorporation

A plasmid standard used in the 2014 UT Austin iGEM team's Expanded Genetic Code Measurement Kit. The plasmid contains an RFP domain fused to a GFP domain by a linker sequence that contains a UAG amber stop codon in the middle for non-canonical amino acid incorporation measurement.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5940
    Illegal XbaI site found at 2474
    Illegal XbaI site found at 3764
    Illegal PstI site found at 1388
    Illegal PstI site found at 1490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5940
    Illegal NheI site found at 1618
    Illegal PstI site found at 1388
    Illegal PstI site found at 1490
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5940
    Illegal BamHI site found at 679
    Illegal BamHI site found at 1456
    Illegal XhoI site found at 1429
    Illegal XhoI site found at 5853
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5940
    Illegal XbaI site found at 2474
    Illegal XbaI site found at 3764
    Illegal PstI site found at 1388
    Illegal PstI site found at 1490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5940
    Illegal XbaI site found at 2474
    Illegal XbaI site found at 3764
    Illegal PstI site found at 1388
    Illegal PstI site found at 1490
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4054


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Categories
Parameters
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