Coding

Part:BBa_K1316018

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-10-16)
Revision as of 21:58, 17 October 2014 by Joanctd.igem (Talk | contribs)

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RBS + csgB curli-forming gene

Curli are extracellular amyloids that form fibers. They are involved in adhesion, cell aggregation and biofilm formation. CsgB is one of the curli-forming proteins. More specifically, it is a subunit that stands at the beginning of each curli fiber anchored to the bacterial membrane.

Characterisation

This part was made to construct BioBricks BBa_K1316013 and BBa_K1316014. For more information about the characterisation of this construct visit the pages of these BioBricks or the Conductive curli module characterisation on our wiki page (http://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/curli/characterisation)!


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 516
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None