Regulatory

Part:BBa_K1400002

Designed by: Dylan Siriwardena   Group: iGEM14_uOttawa   (2014-10-06)
Revision as of 21:56, 17 October 2014 by Dylansiriwardena (Talk | contribs)

PGAL Modified native pGAL

This is the native pGAL1 promoter in S. cerevisiae with its Mig1 sites removed. This is a single input promoter that has four upstream activating sequences (UAS), which are GAL4 binding elements. The Mig1 sites are binding sites for proteins responsible for transcriptional repression of downstream genes to this and other promoters in S. cerevisiae in the presence of glucose. The removal of the Mig1 sites allows transcriptional activation of the promoter in the presence of glucose in the cellular growth medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None