Coding

Part:BBa_K1323009

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-10-04)
Revision as of 21:05, 17 October 2014 by Rkovac31 (Talk | contribs)

DsRed (RFP) Coding Sequence

This is the coding sequence of DsRed_T3 (DNT), a highly fluorescent mutant version of a red fluorescent protein originally found in Discosoma (corals) (Dunn et. al., 2006). It was codon optimized for Staphylococcus aureus by Bose et. al. (2013) and we had this part synthesized by Bio Basic Canada Inc.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.

Dunn, A. K., Millikan, D. S., Adin, D. M., Bose, J. B., and Stabb, E. V. (2006). New rfp- and pES213-Derived Tools for Analyzing Symbiotic Vibrio fisheri Reveal Patterns of Infection and lux Expression In Situ. Applied and Environmental Microbiology. 72 (1): 802-810.


[edit]
Categories
//cds/reporter/rfp
//function/reporter/fluorescence
Parameters
colorRed