Coding

Part:BBa_K1351041

Designed by: Carolin Kobras   Group: iGEM14_LMU-Munich   (2014-10-17)
Revision as of 20:52, 17 October 2014 by LydiaAlzinger (Talk | contribs)

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spaBTC (Modification&Export of subtilin)

The genes spaBTC play a role for the posttranslational modifications and the transport of subtilin. spaB encodes a hydratase, spaC a cyclase, which is responsible for the formation of the thither ring. spaT is coding for a NisT-type ABC-transporter, which exports subtilin, which is later on processed by an unspecific protease (cleavage of leader sequence)


This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2391
    Illegal BamHI site found at 4661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2205
    Illegal AgeI site found at 3213
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 257
    Illegal SapI.rc site found at 2305
    Illegal SapI.rc site found at 4815
    Illegal SapI.rc site found at 6017


[edit]
Categories
Parameters
None