Coding
Part:BBa_K1351041
Designed by: Carolin Kobras Group: iGEM14_LMU-Munich (2014-10-17)
Revision as of 20:52, 17 October 2014 by LydiaAlzinger (Talk | contribs)
spaBTC (Modification&Export of subtilin)
The genes spaBTC play a role for the posttranslational modifications and the transport of subtilin. spaB encodes a hydratase, spaC a cyclase, which is responsible for the formation of the thither ring. spaT is coding for a NisT-type ABC-transporter, which exports subtilin, which is later on processed by an unspecific protease (cleavage of leader sequence)
This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2391
Illegal BamHI site found at 4661 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2205
Illegal AgeI site found at 3213 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 257
Illegal SapI.rc site found at 2305
Illegal SapI.rc site found at 4815
Illegal SapI.rc site found at 6017
[edit]
Categories
Parameters
None |